ASP = aspartic acid ASN = (Asparagine) GLU = glumatic acid GLN = (Glutamine)Nitrous acid causes deamination: transition: C -> T; A -> G 5-Bromouracil (thymine analog)transition: A - T to G - C Acridine: intercalating: frameshifts Alkylator (EMS, a mustard gas) transition: G -> A Ultraviolet
protein secondary structure: alpha helix: wool can stretch beta sheet: silk no stretch beta bend random
Short gene somatostatin (growth hormone-inhibiting hormone): promoter is TATAATG Long gene glucagon (29 amino acids secreted by pancreas and increases the level of blood sugar) (pancreas: convert glycogen to glucose and release from liver) Custom Gene - Enter up to 105 bases
Promoter CTCG TATAATG CGACCAACA
DNA: ATA CTG AGT CTT CAC mRNA: UAU GAC UCA GAA GUG amino acids: TYR ASP SER GLU VAL
TYR ALA ASP SER GLU VAL
ATA CTG ATT CTT CAC UAU GAC UAA GAA GUG TYR ASP STOP
Ala Gly Cys Lys Asn Phe Phe Trp Lys Thr Phe Phe Leu Glu Asn
Index | Lab notes made Nov 07, 2009 by Peter Chen |