22 Computer Simulation of DNA Replication and Expression Notes

ASP = aspartic acid
ASN = (Asparagine)
GLU = glumatic acid
GLN = (Glutamine)

Nitrous acid causes deamination: transition: C -> T; A -> G 5-Bromouracil (thymine analog)transition: A - T to G - C Acridine: intercalating: frameshifts Alkylator (EMS, a mustard gas) transition: G -> A Ultraviolet

protein secondary structure: alpha helix: wool can stretch beta sheet: silk no stretch beta bend random

Short gene somatostatin (growth hormone-inhibiting hormone): promoter is TATAATG Long gene glucagon (29 amino acids secreted by pancreas and increases the level of blood sugar) (pancreas: convert glycogen to glucose and release from liver) Custom Gene - Enter up to 105 bases

Promoter CTCG TATAATG CGACCAACA

DNA: ATA CTG AGT CTT CAC mRNA: UAU GAC UCA GAA GUG amino acids: TYR ASP SER GLU VAL

TYR ALA ASP SER GLU VAL

ATA CTG ATT CTT CAC UAU GAC UAA GAA GUG TYR ASP STOP

Ala Gly Cys Lys Asn Phe Phe Trp Lys Thr Phe Phe Leu Glu Asn


Index Lab notes made Nov 07, 2009 by Peter Chen