gene_cloning2
Tissue Plasminogen Activator (TPA) protein dissolves blood clots in heart attack.
restriction
Palindrome dates: 01/02/2010, 11/02/2011
cloning
This uses hummingbird beta-globin gene, video human gene; beta-galactosidase digets
X-gal.
cDNA
cDNA can be expressed in prokaryotic; shows which genes are expressed; reverse central dogma.
PCR
Primers can be DNA for Dideoxy; Thermus aquaticus: Taq polymerase
HHMI 35:40 PCR
42 Sanger sequencing; 45-52 chip
electrophoresis
Anode is positive terminal
fragment
Dde I (one).
fingerprint
Short Tandem Repeats.
The 3 DNA samples treated by
Southern
blotting w radioactive probes.
termination
Frederick Sanger also Nobel for sequencing insulin.
transplantation
QH442.2 .C743 2002:
Creation#21:00 rat, 23:30 egg donor, Judson's skin cells thru zona pellucida (glycoprotein) - 32:30
QH442.2 .F67 2006:
For spares or repairs.
dolly
Need to reverse epigenetic changes: acetylation of
histones.
===
1=B 2=B? 3=C 4=B 5=A? post-transcriptional processing
6=C 7=E? 8=C? 9=D 10=C? DNA sequencing
- Restriction enzymes: protect against foreign DNA, bacterial DNA have methyl (-CH3) groups:
- Radioactive tritium
(2N + 1P) neutron is turned into a proton and an electron.
- PCR: use Taq polymerase, low replication fidelity; Pfu polymerase(archae) can proofread, but slower
- in Vitro: in an artificial environment outside a living organism.
- Agar agar
(agarose) from Gelidium purpurascens (Rhodophyta), polysaccharide with sulfated galactose monomers.
- Human Genome Project completed 2003: 20-25K genes.
- Genetic markers can be in noncoding DNA, e.g restriction fragment length polymorphisms (RFLPs , pronounced “Rif–lips”).
- Oryza sativa
indica (xian): long/sticky; japonica (geng): short/sticky. javanica: long/NOT sticky.
Fritillaria assyriaca: European lily.
- Celera: Craig Venter; rough draft 2000, 2003 99% complete.
